A DNA-extraction and polymerase-chain-reaction microchip using magnetic beads and thermo-pneumatic valves

Author

Chia, Bonnie T. ; Yang, Xing-Ying ; Cheng, Ming-Yuan ; Yang, Yao-Joe

Author_Institution

Nat. Taiwan Univ., Taipei, Taiwan

fYear

2010

fDate

24-28 Jan. 2010

Firstpage

967

Lastpage

970

Abstract

In this work, we develop a miniaturized system for DNA extraction and polymerase chain reaction (PCR). DNA is extracted from whole blood sample by using silica coated magnetic beads. Electromagnetic actuation is employed to manipulate the magnetic beads for transporting DNA molecules in fluidic channels. Thermo-pneumatic valves with low temperature elevation are integrated into the microfluidic chip for isolating/releasing fluidic samples and reagents. The size of the system is 72mmÃ—54mmÃ—11mm. The device fully operates with a 12V DC voltage and automatically performs DNA extraction and amplification processes after samples and reagents are loaded.

Keywords

DNA; microfluidics; valves; DNA-extraction; amplification processes; electromagnetic actuation; fluidic channels; low temperature elevation; magnetic beads; polymerase-chain-reaction microchip; silica coated magnetic beads; thermopneumatic valves; Coils; DNA; Distributed control; Heating; Magnetic analysis; Microfluidics; Polymers; Purification; Temperature; Valves;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on

Conference_Location

Wanchai, Hong Kong

ISSN

1084-6999

Print_ISBN

978-1-4244-5761-8

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2010.5442369

Filename

5442369