A free-standing and flexible parylene PCR device

Author

Satsanarukkit, Penvipha ; Lo, Hsiwen ; Tai, Yu Chong

Author_Institution

Dept. of Electr. Eng., California Inst. of Technol., Pasadena, CA, USA

fYear

2012

fDate

5-8 March 2012

Firstpage

146

Lastpage

149

Abstract

We present a free-standing and flexible parylene-channel PCR device that allows a simple sealing mechanism to prevent bubbling caused by PCR temperature cycling. This sealing mechanism saves complicated, time-consuming in-channel valve fabrication processes and achieves excellent sealing performance. It is proven experimentally that this new PCR device plus its unique packaging is capable of locking PCR solution in the PCR chamber at 95°C for at least 5 hours without bubbling and leaking. As a result, we have successfully obtained the on chip PCR amplification without the bubble and leaking problem.

Keywords

microfluidics; microsensors; polymers; PCR temperature cycling; bubbling; flexible parylene PCR device; free-standing parylene PCR device; in-channel valve fabrication process; leaking problem; on chip PCR amplification; polymerase chain reaction; sealing mechanism; temperature 95 C; Fluorescence; Heating; Lasers; Microchannel; Nanoelectromechanical systems; Phase change random access memory; Silicon; PCR; bubbling and leaking problems; microfluidics; parylene-C;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2012 7th IEEE International Conference on

Conference_Location

Kyoto

Print_ISBN

978-1-4673-1122-9

Type

conf

DOI

10.1109/NEMS.2012.6196743

Filename

6196743