DocumentCode :
2031908
Title :
The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip
Author :
Lin, Ran ; Chang, Donald C. ; Lee, Yi-Kuen
fYear :
2012
fDate :
5-8 March 2012
Firstpage :
150
Lastpage :
153
Abstract :
Electroendocytosis (EED) is a pulsed-electric-field (PEF) induced endocytosis, facilitating cells uptake molecules through nanometer-sized EED vesicles. We herein investigate the effect of a chemical inhibitor, Cytochalasin D (CD) on the actin-filaments (F-Actin) behavior of single-cell EED. The CD concentration (CCD) can control the depolymerization of F-actin. A multi-chamber micro cell chip was fabricated to study the EED under different conditions. Large-scale single-cell data demonstrated EED highly depends on both electric field and CCD.
Keywords :
adsorption; bioMEMS; biochemistry; bioelectric phenomena; cellular biophysics; lab-on-a-chip; molecular biophysics; polymerisation; proteins; F-actin behavior; actin filaments; biomolecules; chemical inhibitor; cytochalasin D; depolymerization; multichamber microcell chip; pulsed-electric-field induced endocytosis; single-cell electroendocytosis; Fluorescence; Gold; Nanoelectromechanical systems; Polymers; Actin filament; Cytochalasin D; Electroendocytosis; Micro Cell Chip;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2012 7th IEEE International Conference on
Conference_Location :
Kyoto
Print_ISBN :
978-1-4673-1122-9
Type :
conf
DOI :
10.1109/NEMS.2012.6196744
Filename :
6196744
Link To Document :
https://search.ricest.ac.ir/dl/search/defaultta.aspx?DTC=49&DC=2031908
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