Polypyrrole (PPy) nanowire arrays entrapped with glucose oxidase biosensor for glucose detection

Well aligned PPy nanowire arrays with diameter about 20 nm have been accomplished by electrochemical polymerization within anodized aluminum oxide (AAO) template. Glucose oxidase (GOx) was galvanostatically entrapped within the PPy nanoarrays. Scanning electron microscopy (SEM) confirmed the presence of PPy nanowire arrays. The electrochemical properties and response of the PPy-GOx nanowire arrays biosensor to glucose were characterized by cyclic voltammetry and amperometry. Evident current response can be observed in the cyclic voltammogram of the PPy-GOx nanowire arrays biosensor when exposed to glucose in a phosphate buffer solution. Amperometric detection of glucose was successfully achieved and the established optimum conditions with the PPy(GOx) biosensor were 0.4 M pyrrole, 200 U/ml GOx, applied current density of 0.1 mA/cm², polymerization time of 600 s. The biosensor achieved a detection limit of 50 μM, a linear concentration range of 0.1 mM to 8 mM, and sensitivity of 9.97 μA cm^#x2212;2 mM^-1.