Control of cell elution from a dielectrophoresis micro-array using a permittivity gradient

Author

Lee, R.S. ; Arnold, W.M. ; Pethig, R.

Author_Institution

Univ. of Wales, Bangor, UK

fYear

2004

fDate

17-20 Oct. 2004

Firstpage

352

Lastpage

355

Abstract

Microparticles, e.g. biological cells, can be selectively trapped or released from a dielectrophoretic (DEP) electrode array by manipulation of the relative permittivity of the suspending medium. Frequency windows are predicted in which the DEP force decreases, increases or even reverses polarity as a function of medium permittivity, depending on the type of organism. Experimental validation is presented for yeast cells which showed progressive release as the relative permittivity was reduced from 130 to 78. Quantification of DEP collection used a combination of dark-field microscopy with image processing.

Keywords

cellular biophysics; electrophoresis; image processing; optical microscopy; permittivity; biological cells; cell elution control; dark-field microscopy; dielectrophoresis microarray; dielectrophoretic electrode array; frequency dependent DEP force; image processing; microparticles; permittivity gradient; selective release; selective trapping; suspending medium relative permittivity manipulation; yeast cells; Biomembranes; Conductivity; Containers; Dielectrophoresis; Electrodes; Frequency; Fungi; Mathematical model; Microscopy; Permittivity;

fLanguage

English

Publisher

ieee

Conference_Titel

Electrical Insulation and Dielectric Phenomena, 2004. CEIDP '04. 2004 Annual Report Conference on

Print_ISBN

0-7803-8584-5

Type

conf

DOI

10.1109/CEIDP.2004.1364260

Filename

1364260