Reproducibility investigations and different experiments with a new type of electronically-conducting-polymer based bipotentiostatic uric acid sensor

Reproducibility investigations and different experiments with a new type of electronically-conducting-polymer based bipotentiostatic uric acid sensor have been made. The sensor was constructed by using dodecyl sulfate doped poly-N-methyl-pyrrole for enzyme immobilization. This polymer film can incorporate the enzyme in one step during its electropolymerisation. By the bipotentiostatic measuring method the two working electrodes (with and without the enzyme) are analogously prepared and polarized and the currents in the two circuits are measured parallel in time. The layout, which consists of 4 electrode surfaces (W1, W2, Ref, Counter) was prepared by thick film technology on sintered alumina ceramic substrates. The reproducibility of the characteristics using various electrode materials (Au, Pt), and the influence of the temperature or the current density of the polymerisation have been investigated. Different pretreatment of the working electrodes have been applied. Comparison of two type of uricase enzymes have been made.