Analyzing the dynamic range of fluorescent detection in microarray scanner

Author

Liqian, Wang ; Zukang, Lu

Author_Institution

Nat. Eng. & Technol. Res. Center for Opt. Instrum., Zhejiang Univ., Hangzhou

fYear

2006

fDate

16-18 Oct. 2006

Firstpage

136

Lastpage

138

Abstract

By analyzing the dark current noise of photomultiplier tube (PMT) and the thermal noise of feedback resistor, the detection limit of the processing circuit in the microarray scanner is calculated theoretically, and the saturated signal of that is obtained by the anode radiant sensitivity of the PMT under optimized gain. The dynamic range is the saturated signal divided by the detection limit, which is 8773 in theory. The experiment result was acquired after scanning a fluorescent calibration slide by the scanner, which was 4096.

Keywords

biochemistry; fluorescence; lab-on-a-chip; molecular biophysics; photomultipliers; proteins; DNA microarray; anode radiant sensitivity; dark current noise; feedback resistor; fluorescent calibration slide; fluorescent detection; microarray scanner; photomultiplier tube; protein microarray; thermal noise; Circuit noise; Dark current; Dynamic range; Feedback circuits; Fluorescence; Photomultipliers; Resistors; Signal analysis; Signal processing; Thermal resistance;

fLanguage

English

Publisher

ieee

Conference_Titel

Biophotonics, Nanophotonics and Metamaterials, 2006. Metamaterials 2006. International Symposium on

Conference_Location

Hangzhou

Print_ISBN

0-7803-9773-8

Electronic_ISBN

0-7803-9774-6

Type

conf

DOI

10.1109/METAMAT.2006.335017

Filename

4134756