The Biocompatibility Study of Fe3O4 Magnetic Nanoparticles Used in Tumor Hyperthermia

To evaluate the biocompatibility of self-prepared Fe₃O ₄ magnetic nanoparticles, which has the potential application in tumor hyperthermia, MTT assay was used to evaluate the in vitro cytotoxicity; hemolysis test was carried out to estimate its blood toxicity; Fe₃O₄ suspended in sterile 0.9%NaCl were intraperitoneally injected into KM mouse to calculate the LD₅₀; micronucleus (MN) were reckoned to identify its genotoxicity. The experiments showed that the cytotoxicity of the material on mouse fibroblast (L-929) cell lines was between 0~1 degree; it has no hemolysis activity; its LD₅₀ arrived at 7.57g/kg after intraperitoneal injection of 1ml suspension; micronucleus test showed it has no genotoxic effects either. The results indicated that the self-prepared nanosized Fe₃O₄ is a kind of high biocompatibility materials and suitable for further application in tumor hyperthermia