Characterization of immobilized pH gradient formed in capillary based on quantum dots

Author

Xie, Yao ; Xie, Haiyan ; Qu, Feng ; Luo, Aiqin ; Geng, Lina ; Deng, Yulin

Author_Institution

Beijing Inst. of Technol., Beijing

fYear

2007

fDate

23-27 May 2007

Firstpage

1063

Lastpage

1066

Abstract

A new fluorescence imaging method was used for the characterization of the immobilized pH gradient formed in capillary, which was based on charge carried quantum dots (QDs). It was found that the positive charged QDs were concentrated in the same positive region in capillary after CIEF, no matter which terminal of the capillary was used for injection. Furthermore, the formation of the pH gradient and their effect in the practical separation was tested by BSA enrichment experiment, which showing that the sensitivity of detection of BSA was improved remarkably compared with capillary zone electrophoresis (CZE).

Keywords

biological techniques; electrophoresis; fluorescence spectroscopy; molecular biophysics; pH measurement; semiconductor quantum dots; BSA enrichment experiment; CIEF; bovine serum albumin; capillary isoelectric focusing; capillary zone electrophoresis; charge carried quantum dots; fluorescence imaging method; immobilized pH gradient characterization; Chemical analysis; Chemical products; Coatings; Electrokinetics; Electrons; Fluorescence; Nuclear magnetic resonance; Quantum dots; Spectroscopy; Surface morphology;

fLanguage

English

Publisher

ieee

Conference_Titel

Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-1077-4

Electronic_ISBN

978-1-4244-1078-1

Type

conf

DOI

10.1109/ICCME.2007.4381903

Filename

4381903