A Study on Real Time Estimation Viability of Cells Based on DIC

Based on the morphological differences between normal, apoptosis and necrosis cell, a method on real time estimation viability of cells based on DIC (differential interference contrast) microscopy is presented in the paper. The Jurkat cell samples were used for investigation the relationship between morphological parameters and cell viabilities in the paper. According to the scatter charts of Jurkat cell roundness, radius ratio and area, the cell areas were mainly distributed from 40 mum² to 80 mum². And the areas of apoptosis and necrosis cell were distributed in other range, i.e. larger areas or fewer areas. Not any chemical medicaments need to add in the samples by the method presented in the paper in order to get the viability of cells. The survival rate of Jurkat cells and the percentages of death cells in different death modes after ALA-PDT was estimated by the method presented in the paper. The experiment results showed that not only the results of survival rate of Jurkat cells estimated by the method presented in the paper were consistent with the results estimated by accepted MTT method, but also the percentages of death cells in different death modes can be got on real time by the method presented in the paper.