Title :
Evaluation of a Rat Model of Parkinson´s Disease by Injection of 6-OUDA into the Substantia Nigra
Author :
Mao, Jian ; Yang, Wei ; Wang, Ran ; Luan, Yujing ; Qing, Hong ; Deng, Yulin
Author_Institution :
Beijing Inst. of Technol., Beijing
Abstract :
A rat model of Parkinson´s disease was established by direct injection of 6-OHDA into the substantia nigra unilaterally. Animals were assessed by D-amphetamine rotation test, anti-TH staining and detection of dopamine and its metabolites by HPLC-ECD. Rotational behavior experiment shown that the achievement ratio of PD model is 48.9%. Anti-TH staining result indicated that 6-OHDA treatment caused extensive loss of TH immunoreactivity in the substantia nigra and their projections to the striatum. Moreover, DA and its metabolites reduced remarkably in the substantia nigra and striatum of 6-OHDA lesioned rat model compared to control. Therefore, by evaluation of experimental animals, unilateral 6-OHDA induced substantia nigra degeneration can establish dependable rat model of Parkinson´s disease with higher achievement ratio.
Keywords :
diseases; neurophysiology; 6-OHDA; D-amphetamine rotation test; HPLC-ECD; Parkinson´s disease; dopamine; immunoreactivity; metabolites; rat model; substantia nigra; Animals; Chemical analysis; Needles; Neurons; Organic chemicals; Parkinson´s disease; Radio access networks; Rats; Skull; Tin;
Conference_Titel :
Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-1077-4
Electronic_ISBN :
978-1-4244-1078-1
DOI :
10.1109/ICCME.2007.4381977
