On-chip electroporation: Characterization, modeling and experimental results

In this paper, we describe an on-chip electroporation (EP) method for high precision nano-injection of bio-molecules into single cells. EP is an electrical stimulation method to create nano-pores on the cell plasma membrane. Herein, we first put forward the computational models of the cultured cells microelectrodes. We thereafter discuss practical considerations by demonstrating the preliminary experimental results. The mouse fibroblast cells are cultured above electrodes while experiencing a low frequency (10 Hz) electrical field (EF) in the presence of propidium iodide (PI).