Title :
A PPY/PT for assaying L-asparaginase activity in Proteus vulgaris the construction and application of the piezoelectric transducer
Author :
Ren, Jiali ; Liu, Zhenhua
Author_Institution :
Hunan Key Lab. of Grain & Oil Process. & Quality Control, Central South Univ. of Forestry Technol., Changsha, China
Abstract :
The polypyrrole / piezoelectric transducer (PPY/PT) was constructed to assay L-asparaginase activity. The initial reaction rate had a linear relationship with the activity of L-asparaginase in the range of 0.01U·ml-1~0.10 U·ml-1. The detection limit was 0.01 U·ml-1. The proposed method was used to determine L-asparaginase activity in Proteus vulgaris from different strains. Compared with Nesslerization method, proposed method was accurate, simple, non-toxic and real-time.
Keywords :
drugs; pharmaceutical industry; piezoelectric transducers; L-asparaginase drug activity; Proteus vulgaris; polypyrrole-piezoelectric transducer; Biochemistry; Calibration; Films; Monitoring; Polyimides; Probes; Substrates; L-asparaginase; NH3; PPY/PT; Proteus vulgaris; activity;
Conference_Titel :
New Technology of Agricultural Engineering (ICAE), 2011 International Conference on
Conference_Location :
Zibo
Print_ISBN :
978-1-4244-9574-0
DOI :
10.1109/ICAE.2011.5943923
