• DocumentCode
    2091053
  • Title

    Calculation and Simulation of Temperature in Real-time PCR Instrument

  • Author

    Liu, C.B. ; Feng, J.H.

  • Author_Institution
    Beijing Univ. of Technol., Beijing
  • fYear
    2007
  • fDate
    23-27 May 2007
  • Firstpage
    1569
  • Lastpage
    1572
  • Abstract
    In this paper, we designed a model of the PCR reaction chamber by ourselves and calculated the heat flux of the reaction chamber, the lid and the sample. On the basis of the temperature calculation we simulated and analyzed the temperature field´s dynamic distribution of the reaction system and we find that the simulative result and the calculating result match each other. This offers an academic foundation to develop a self-design real-time PCR instrument.
  • Keywords
    chemical reactors; fluorescence; heat transfer; molecular biophysics; temperature distribution; thermodynamics; PCR reaction chamber; fluorescence; heat flux; polymerase chain reaction; real-time PCR instrument; temperature calculation; temperature dynamic distribution; temperature simulation; Analytical models; Biomedical engineering; Fluorescence; Heat engines; Instruments; Paper technology; Plastics; Polymers; Real time systems; Temperature distribution;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on
  • Conference_Location
    Beijing
  • Print_ISBN
    978-1-4244-1077-4
  • Electronic_ISBN
    978-1-4244-1078-1
  • Type

    conf

  • DOI
    10.1109/ICCME.2007.4382010
  • Filename
    4382010