Title :
Calculation and Simulation of Temperature in Real-time PCR Instrument
Author :
Liu, C.B. ; Feng, J.H.
Author_Institution :
Beijing Univ. of Technol., Beijing
Abstract :
In this paper, we designed a model of the PCR reaction chamber by ourselves and calculated the heat flux of the reaction chamber, the lid and the sample. On the basis of the temperature calculation we simulated and analyzed the temperature field´s dynamic distribution of the reaction system and we find that the simulative result and the calculating result match each other. This offers an academic foundation to develop a self-design real-time PCR instrument.
Keywords :
chemical reactors; fluorescence; heat transfer; molecular biophysics; temperature distribution; thermodynamics; PCR reaction chamber; fluorescence; heat flux; polymerase chain reaction; real-time PCR instrument; temperature calculation; temperature dynamic distribution; temperature simulation; Analytical models; Biomedical engineering; Fluorescence; Heat engines; Instruments; Paper technology; Plastics; Polymers; Real time systems; Temperature distribution;
Conference_Titel :
Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-1077-4
Electronic_ISBN :
978-1-4244-1078-1
DOI :
10.1109/ICCME.2007.4382010
