Title :
Preparation and characterization of skin secretion hydrolysates from giant salamander (Andrias davidianus)
Author :
Qu, Min ; Wang, Wenli ; Li, Wei ; Yu, Xinying ; Tong, Changqing ; Kong, Liang ; Jin, Qiao ; Feng, Xuqiao
Author_Institution :
Coll. of Food Sci., Shenyang Agric. Univ., Shenyang, China
Abstract :
By using Aspergillus sp. acid protease, the preparation of skin secretion hydrolysates from giant salamander (Andrias davidianus) was studied. Through orthogonal test, the optimum hydrolytic condition as follows: temperature 55°C, pH2.0, E/S value is 0.03%, and hydrolysis time 3h. MALDI-TOF mass spectrometry showed that molecular weights of hydrolysates were below 3.5kDa.
Keywords :
MALDI mass spectra; food processing industry; skin; Andrias davidianus; Aspergillus sp. acid protease; MALDI-TOF mass spectrometry; food protein; giant salamander; molecular weight; orthogonal test; skin secretion hydrolysate preparation; temperature 55 C; time 3 h; Educational institutions; Ocean temperature; Peptides; Production; Proteins; Skin; Spectroscopy; acid protease; giant salamander; hydrolysate; skin secretion;
Conference_Titel :
New Technology of Agricultural Engineering (ICAE), 2011 International Conference on
Conference_Location :
Zibo
Print_ISBN :
978-1-4244-9574-0
DOI :
10.1109/ICAE.2011.5943941
