Glycerol diffusion in skin at glucose impact on tissue

In this paper we present results of experimental study of glucose impact on skin tissue by testing tissue structure alteration at glycerol diffusivity. The measurements have been performed using Ocean Optics spectrometer USB4000 in transmittance mode. In the in vitro experiments twenty samples of intact rat skin were used. Transmittance of ten samples was measured immediately after obtaining the samples concurrently with administration of 58% aqueous glycerol solution in the spectral range 400-1000 nm. The second group of the rat skin samples (ten samples) was incubated during 24 hours in 40%-glucose solution and during 24 hours in physiological solution. After that transmittance of the ten samples was measured concurrently with administration of the 58%- glycerol solution. Special computer program has been developed for processing of the experimental data and estimation of the glycerol diffusion rate. Degree of optical clearing of intact skin samples and skin samples previously immersed in glucose solution were estimated and compared. As a result we found that the optical clearing of intact skin increases about 3 times faster in comparison with skin immersed in the glucose solution.