Assay for Affinity of Proteins onto Model Biomembrane

In this paper, immobilized artificial membrane (IAM) was selected as the model biomembrane, and packed into capillary column as stationary phase of liquid chromatography to screen the proteins having strong interaction with biomembrane rapidly. The affinity between IAM and ten common proteins (pepsin, bovine serum albumin, casein, transferring, myosin, insulin, hemoglobin, cytochrome C, trypsin, lysozyme), whose pI ranged from 2.0-11.5, were analyzed in different pH mobile phase including acidic, neutral and alkaline buffer. The optimized condition was obtained for the affinity of IAM and proteins. The proteins having strong affinity with IAM were screened out under each pH mobile phase. It can provide the experimental foundation for proteins to be immobilized onto biomembrane to study the membrane related interaction, separation, protein bioactivity, and drug screening and so on.