HPCE analysis of the effect of Sargcassumfusiforme polysaccharides on the complex mobility of erythrocytes in tumor-bearing organisms

This study was to analyze the effect of Sargassum fusiforme polysaccharides (SFPS) on the electrophoretic mobility of erythrocytes in tumor-bearing organisms. A tumor mouse model was developed, and the mice were treated with high, medium, and low dosages of SFPS through abdominal injection for 7 d. Erythrocytes were collected and prepared into suspensions, and the complex motilities of the cells were measured using high performance capillary electrophoresis (HPCE). Experimental conditions: capillaries: 75 mum times 50 cm; buffer for electrophoresis: phosphate solution containing HPMC (0.1 mol/L, pH 7.4); injection pressure: 3.448 kPa; injection time: 10 s; separation voltage: 20 kV; column temperature: 25degC. The electroosmotic mobility was found to be 2.16 times 10^-4 cmldrV^-1ldrs^-1, so SFPS can decrease the migration time of erythrocytes in tumor-bearing organisms, which was 17.56´ for the control group, 13.96´ for the low-dosage group, 12.9´ for the medium-dosage group, 13.51´ for the high-dosage group, while that for the normal group was 11.51´; and increase its complex mobility, which was 1.06 times 10^-4 cm²ldrV^-1ldrs^-1 for the control group, 1.19 times 10^-4 cm²ldrV^-1ldrs^-1 for the low dosage group, 1.29 times 10^-4 cm²ldrV^-1ldrs^-1 for the middle dosage group, 1.23 times 10^-4 cm²ldrV^-1ldrs^-1 for the high dosage group, while that for the normal group was 1.45 times 10^-4 cm²ldrV^-1ldrs^-1. The erythrocyte mobility for all three dosage groups differ significantly from that for the negative control (P < 0.01). SFPS can change the complex mobility of erythrocytes from tumor-bearing organisms toward the level for erythrocytes from normal organisms, and this may be because it can change the density of the surface - charge on erythrocytes. So HPCE can be used as auxiliary tool for determining the physiological state and functions of erythrocytes.