Title :
Analyses of PAis Metabolites Using a Peroxynitrite/Fe(III) porphyrin Biomimetic System by HPLC
Author :
Luo, Yunjing ; Liu, Rui ; She, Yuanbin ; Zhong, Rugan
Author_Institution :
Beijing Univ. of Technol., Beijing
Abstract :
The peroxynitrite/Fe(III)porphyrin chemical system was established firstly to investigate the effects of peroxynitrite on the metabolism of polycyclic aromatic hydrocarbons(PAHs). By comparing the conversion ratio of benzo[a]pyrene (B[a]P) in four model systems consisting of different iron porphyrins, we found that the peroxynitrite/T(p-Cl)PPFeCl system showed the highest catalytic efficiency, which conversion ratio of B[a]P reached 20.71%. We also analyzed the metabolites of B[a]P and pyrene (PN) produced from this system by HPLC and MS methods, and successfully identified the formation of nitrobenzo [a] pyrene and nitropyrene which are the special metabolites induced by peroxynitrite.
Keywords :
biochemistry; biomimetics; catalysis; chromatography; organic compounds; HPLC; PAH metabolite; benzo-a-pyrene conversion ratio; benzo-a-pyrene metabolites; high performance liquid chromatography; iron porphyrins; mass spectrometry; nitrobenzo-a-pyrene; nitropyrene; peroxynitrite-Fe(III)porphyrin biomimetic system; polycyclic aromatic hydrocarbons; Biochemistry; Biomedical engineering; Biomimetics; Chemical engineering; Chemical technology; Educational institutions; Iron; Methanol; Performance analysis; Power engineering and energy;
Conference_Titel :
Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-1077-4
Electronic_ISBN :
978-1-4244-1078-1
DOI :
10.1109/ICCME.2007.4382045
