Title :
Enhance the resolution of STED by fluorescent lifetime subtractive imaging
Author :
Xiang Hao ; Cuifang Kuang ; Zhaotai Gu ; Shuai Li ; Yifan Wang ; Xu Liu
Author_Institution :
State Key Lab. of Modern Opt. Instrumentations, Zhejiang Univ., Hangzhou, China
Abstract :
By the time-gated detection technique, combined with the principle of virtual adaptable aperture based subtractive imaging, we achieve a horizontal resolution below 80 nm with the incident power of continuous-wave STED beam equaling 50 mW.
Keywords :
fluorescence; image enhancement; image resolution; optical microscopy; stimulated emission; continuous-wave STED beam; fluorescent lifetime subtractive imaging; horizontal resolution; incident power; resolution enhancement; time-gated detection; virtual adaptable aperture based subtractive imaging;
Conference_Titel :
Communications and Photonics Conference (ACP), 2012 Asia
Conference_Location :
Guangzhou
Print_ISBN :
978-1-4673-6274-0
