Determination of free amino acids and taurine in Sinonova- culaconstricta with 2, 4-dinitrochlorobenzene precolumn derivatization

A method was established to determination the 18 kinds of free amino acids and the taurine in Sinonovacula constricta by HPLC with precolumn derivatization with 2,4-dinitrochlorobenzene as a derivatization agent, A Kormat uinversil C18 column was used with gradient elution at the detection wavelength of 360 nm. There is a good linear relationship between the peak areas and concentration in the range of 1~50 μmol/L of the 18 kinds of free amino acids and taurine.The average recovery is 93.7%~110.3%, The detection limit is 0.36~0.86 μmol/L and RSD is 1.01%~4.46%.