Title :
Visible spectrophotometric determination of total flavonoids in Buddleja officinalis Maxim
Author_Institution :
Jiangsu Key Lab. of Marine Biotechnol., Huaihai Inst. of Technol., Lianyungang, China
Abstract :
Rutin was used as the standard sample and NaNO2, Al (NO3)3 and NaOH was used to compose the coloration system, the visible spectrophotometric assay of determination of total flavonoids content in Buddleja officinalis Maxim was built. The results showed that the measure wavelength was 510 nm, the total flavonoids content in Buddleja officinali Maxim was an average of 20.31%, the average recovery was 100.3% and RSD was 0.75%.
Keywords :
agricultural engineering; aluminium compounds; colour; sodium compounds; spectrophotometry; Al(NO3)3; Buddleja officinalis maxim; NaNO2; NaOH; coloration system; flavonoids; loganiaceae buddleja plant; rutin; visible spectrophotometric assay; Aluminum; Electron tubes; Ethanol; Instruments; Laboratories; Mathematical model; Wavelength measurement; Buddleja officinalis Maxim; deternination; spectrophotometry; total flavonoids;
Conference_Titel :
New Technology of Agricultural Engineering (ICAE), 2011 International Conference on
Conference_Location :
Zibo
Print_ISBN :
978-1-4244-9574-0
DOI :
10.1109/ICAE.2011.5943985
