Title :
Single molecule tracking of P-glycoprotein in live cells reveals dynamic heterogeneity
Author :
Cheney, P.P. ; Stachler, M.D. ; Knowles, M.K.
Author_Institution :
Dept. of Chem. & Biochem., Univ. of Denver, Denver, CO, USA
fDate :
Aug. 28 2012-Sept. 1 2012
Abstract :
P-glycoprotein transports chemotherapy drugs from the plasma membrane and allows cancer cells to survive treatment. We transiently transfected PGP labeled with enhanced green fluorescent protein (PGP-EGFP) into MES-SA cells and used single molecule tracking techniques to characterize the dynamics on the surface of live cells. PGP exhibits freely diffusive behavior at short times and is confined at long times with a transition to anomalous diffusion at 0.7 s.
Keywords :
biodiffusion; biological techniques; biomembrane transport; cancer; drugs; fluorescence spectroscopy; molecular biophysics; proteins; EGFP label; MES-SA cells; P-glycoprotein single molecule tracking; PGP transfection; PGP-EGFP; anomalous diffusion; cancer cells; chemotherapy drug transport; dynamic heterogeneity; enhanced green fluorescent protein; freely diffusive behavior; live cell surface dynamics; live cells; plasma membrane; Biological system modeling; Electronic mail; Fluorescence; Image segmentation; Tracking; Cell Line; Green Fluorescent Proteins; Humans; Microscopy, Confocal; Microscopy, Fluorescence; P-Glycoprotein;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2012 Annual International Conference of the IEEE
Conference_Location :
San Diego, CA
Print_ISBN :
978-1-4244-4119-8
Electronic_ISBN :
1557-170X
DOI :
10.1109/EMBC.2012.6346635
