Growth Inhibition and Induction of Apoptosis in Sarcoma 180 Cells by Juglone in Vivo

To study the effect of growth inhibition and induction of apoptosis in sarcoma 180 cells by juglone in vivo. S₁₈₀ tumor-bearing mice were set up to investigate the effects of juglone on tumor growth inhibition and apoptosis. After HE staining, the pathomorphologic changes of tumor cells were observed by optical microscope. The ultrastructural changes of tumor cells were observed by transmission electron microscopy. Flow cytometry was used to observe the apoptosis rate in S₁₈₀ cells. The experimental results showed that the tumor inhibition rate at the dosage of 2, 4 and 8mumol/kg of juglone was 29.04%, 37.33%, 52.66% respectively. The tumor weight of all dose groups of juglone were lower significantly than that in blank control group (P<0.01). The typical morphological changes of apoptosis were observed, such as chromatin condensation, margination against nuclear envelope and formation of apoptotic bodies by transmission electron microscopy. The subdiploid peak was found and the apoptosis rate of juglone at the dosage of 2, 4 and 8 mumol/kg was 5.58plusmn0.63%, 6.66plusmn0.85%, 10.27plusmn1.05% respectively. Juglone can inhibit the growth and induce apoptosis of sarcoma 180 cells in vivo.