DocumentCode :
2116665
Title :
Fast cell immobilization by using non-immunological method for cell based biosensor
Author :
Park, Jihwang ; Müller, Michael ; Jang, Eunji ; Kim, Jungtae ; Hong, Lan-Young ; Kim, Dong-Pyo ; Seidel, Helmut ; Koh, Won-Gun
Author_Institution :
Interdiscipl. Human Biotechnol., Korea Inst. of Sci. & Technol. Eur. GmbH, Saarbrücken, Germany
fYear :
2010
fDate :
1-4 Nov. 2010
Firstpage :
1932
Lastpage :
1935
Abstract :
This paper reports a concept of a micro cell analysis system for fast cell immobilization by using a non immunological method which is composed of a cell/drug inlet, a sheath flow inlet, a micro chamber and a outlet to biosensor/waste. Micro cell wells were located in the micro chamber. To define optimized parameters of micro cell analysis system, cell adhesion experiment using Hela at 0.5×106, 1.0×106 or 2.0×106 cells/ml in micro wells with 10μm depth and 25μm, 50μm and 100μm size respectively was performed. Different tendencies of cell adhesion between 0.5×106 cells/ml and 1.0×106 cells/ml concentration were not detected. The highest concentration gave the best performance of cell immobilization, even though cell congregation appeared.
Keywords :
adhesion; biological techniques; biosensors; cellular biophysics; biosensor; cell adhesion; cell immobilization; depth 10 mum; microcell analysis; microchamber; non-immunological method; size 100 mum; size 25 mum; size 50 mum;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Sensors, 2010 IEEE
Conference_Location :
Kona, HI
ISSN :
1930-0395
Print_ISBN :
978-1-4244-8170-5
Electronic_ISBN :
1930-0395
Type :
conf
DOI :
10.1109/ICSENS.2010.5689990
Filename :
5689990
Link To Document :
بازگشت