DocumentCode :
2122632
Title :
Phage Langmuir-Blodgett films for biosensing applications
Author :
Olsen, Eric ; Guntupalli, Rajesh ; Sorkulova, Iryna ; Long, Robert ; Neely, William ; Vodyanoy, Vitaly
Author_Institution :
Clinical Res. Lab., Keesler AFB, MS, USA
fYear :
2010
fDate :
1-4 Nov. 2010
Firstpage :
401
Lastpage :
406
Abstract :
The microstructure and functionality of methicillin-resistant Staphylococcus aureus (MRSA) biosensors prepared from novel Langmuir-Blodgett monolayers of lytic bacteriophage were characterized using scanning imaging ellipsometry (SIE) and scanning electron microscopy (SEM). SEM revealed uniform distribution of phage monolayers immobilized to biosensor substrates. SIE indicated monolayers were 49.8 ± 18.3 nm thick on average. SIE charged couple imaging analysis of biosensors yielded an average intensity of 159±7 and 194±13 for 108 and 109 CFU/ml MRSA concentrations, respectively.
Keywords :
Langmuir-Blodgett films; biosensors; ellipsometry; microorganisms; molecular biophysics; monolayers; scanning electron microscopy; Langmuir-Blodgett films; SEM; SIE; biosensors; functionality; lytic bacteriophage; methicillin-resistant Staphylococcus aureus; microstructure; scanning electron microscopy; scanning imaging ellipsometry; size 49.8 nm;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Sensors, 2010 IEEE
Conference_Location :
Kona, HI
ISSN :
1930-0395
Print_ISBN :
978-1-4244-8170-5
Electronic_ISBN :
1930-0395
Type :
conf
DOI :
10.1109/ICSENS.2010.5690208
Filename :
5690208
Link To Document :
https://search.ricest.ac.ir/dl/search/defaultta.aspx?DTC=49&DC=2122632
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