DocumentCode :
2125319
Title :
Label-free biosensing using a nanostructured Fabry-Perot interferometer
Author :
Zhang, Tianhua ; Karandikar, Sukrut ; Gong, Zhongcheng ; Giorno, Rebecca ; Que, Long
Author_Institution :
Instn. for Micromanufacturing, Louisiana Tech Univ., Ruston, LA, USA
fYear :
2010
fDate :
1-4 Nov. 2010
Firstpage :
2065
Lastpage :
2068
Abstract :
This paper reports a novel label-free biosensor based on a nanostructured Fabry-Perot interferometer (FPI) for the first time. Au-coated nanostructure layer inside the FPI offers significantly enhanced optical interference signals due to localized surface Plasmon resonance (SPR) effect. Immobilization of capture protein, Protein A, on the nanostructure layer and its binding with immunoglobulin G (IgG) inside the FPI are monitored in real time, resulting in interference fringes shift of the signals. Fourier transform (FT) is performed on the measured reflectance interference spectrum. Only a single peak whose X-axis position is the effective optical thickness (EOT) of nanostructured layer is presented for each spectrum, which simplifies the quantified analysis of the signals. Current results show that the detection-of-limit (DOL) of the biosensor should be much lower than 5ng/ml.
Keywords :
Fabry-Perot interferometers; biochemistry; biosensors; bonds (chemical); gold; light interference; molecular biophysics; nanobiotechnology; nanosensors; nanostructured materials; proteins; surface plasmon resonance; Au; Fourier transform; binding; capture protein; detection-of-limit; effective optical thickness; gold-coated nanostructure layer; immunoglobulin G; interference fringes; label-free biosensing; localized surface plasmon resonance effect; nanostructured Fabry-Perot interferometer; optical interference signals; protein A; protein Immobilization; reflectance interference spectrum;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Sensors, 2010 IEEE
Conference_Location :
Kona, HI
ISSN :
1930-0395
Print_ISBN :
978-1-4244-8170-5
Electronic_ISBN :
1930-0395
Type :
conf
DOI :
10.1109/ICSENS.2010.5690317
Filename :
5690317
Link To Document :
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