The Effect of Cyclic Stretch on Apoptosis of Human Squamous Carcinoma of Tongue Cell Line Tca8113

The effects of cyclic stretch on apoptosis of human squamous carcinoma of tongue cell line Tca8113 were studied by Flexercell-4000 strain unit, examined and analyzed by flow cytometry. Tca8113 cells were grown on six-well culture plates and loaded by different regimen(different waveform and elongation amplitude). Results: The apoptosis rate increased significantly in experimental groups when compared with the control group and the apoptosis rate increased significantly with the stretch amplitude (P<0.05). With 5 %, 10 % elongation amplitude, different waveform had different apoptosis rate (P<0.05). With 15 % elongation amplitude, the apoptosis rate increased significantly in square wave group when compared with sine wave group and triangle wave group (P<0.05), there was no significant difference in apoptosis rate between triangle wave group and sine wave group. It was indicated that cyclic stretch could induce the apoptosis of Tca8113 cells, the waveform and elongation amplitude play important roles in apoptosis.