The effect of squarewave stretching on apoptosis of Human oral Squamous Cell Carcinoma KB Cells

Author

Hua Qu ; Peng Gao

Author_Institution

Coll. of Sci., Tianjin Polytech. Univ., Tianjin, China

fYear

2012

fDate

16-18 Oct. 2012

Firstpage

1598

Lastpage

1601

Abstract

Using FX-4000 strain unit, the apoptosis of Human oral Squamous Cell Carcinoma KB Cells that underwent different mechanical strain were studied. Flow Cytometer was adopted to investigate the apoptosis of Human oral Squamous Cell Carcinoma KB Cells. Experimental results indicated that cell apoptosis rate increased significantly after KB cells were subjected to squarewave with 10% or 15% elongation at frequency 0.5Hz for 24h, the cell apoptosis rate of squarewave (15% elongation at frequency 0.5Hz for 24h) group increased significantly compared with squarewave (10% elongation at frequency 0.5Hz for 24h) group. It was indicated that cyclic stretch could promote the apoptosis of KB cells, the waveform and elongation amplitude play important roles in promoting apoptosis.

Keywords

biomechanics; cancer; cellular biophysics; elongation; waveform analysis; FX-4000 strain unit; cell apoptosis rate; cyclic stretch; elongation amplitude; flow cytometer; frequency 0.5 Hz; human oral squamous cell carcinoma KB cell apoptosis; mechanical strain; squarewave stretching; time 24 hr; waveform; Human oral Squamous Cell Carcinoma KB Cells; apoptosis; cyclic stretch; squarewave;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2012 5th International Conference on

Conference_Location

Chongqing

Print_ISBN

978-1-4673-1183-0

Type

conf

DOI

10.1109/BMEI.2012.6512975

Filename

6512975