Title :
Highly sensitive surface-enhanced raman nano-probing for direct proteomic profiling
Author :
Chen, Yi ; Xu, Zhida ; Liu, Logan ; Coppe, Jean-Philippe
Author_Institution :
Dept. of Electr. & Comput. Eng., Univ. of Illinois at Urbana-Champaign, Urbana, IL, USA
Abstract :
Nanocone structures were fabricated by a unique etching-passivation process as the substrates for Surface Enhanced Raman Spectroscopy (SERS). This novel nanostructured SERS substrate allowed reaching unprecedented detection limits (fM of Rhodamin6G; pM of crude peptides), and supported the detection of subtle changes in peptide probe sequences, such as single amino acid phosphosphorylation or mutation. Thus, surface-conjugated peptides could be used as highly potent biological nano-sensors for sensitive profiling of specific protein mediated chemical changes on nano-sensing probes. We hope such kinase-enzyme activity screening array device and technol ogy will advance all aspects of Life Sciences, and potentially be applied to environment monitoring.
Keywords :
biosensors; enzymes; etching; molecular biophysics; molecular configurations; nanosensors; passivation; proteomics; surface enhanced Raman scattering; Rhodamin6G; biological nanosensors; crude peptides; direct proteomic profiling; environment monitoring; etching-passivation process; highly sensitive surface-enhanced Raman nanoprobing; kinase-enzyme activity screening array device; mutation; nanocone structures; nanosensing probes; nanostructured SERS substrate; peptide probe sequences; single amino acid phosphosphorylation; surface enhanced Raman spectroscopy; surface-conjugated peptides;
Conference_Titel :
Sensors, 2010 IEEE
Conference_Location :
Kona, HI
Print_ISBN :
978-1-4244-8170-5
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2010.5690802
