Microscopic measurement using digital image restoration

The image deconvolution technique was applied in micro-measurement. By fabricating a pointolite, the point spread function of the optical system is measured. After using the maximum likelihood estimation algorithms and iteration threshold segmentation algorithms, the geometric blurring in traditional microscopy is removed. The technique is applied to measure areas of scratches scaled in 10 microns, which affect the surface properties of medical department of orthopedics plates. The technique makes great sense in the development of corresponding measurement.