Title :
Direct nuclear delivery of DNA macromolecules using the photothermal nanoblade
Author :
Ting-Hsiang Wu ; Yi-Chien Wu ; Sagullo, Enrico ; Teitell, Michael A. ; Chiou, Pei-Yu Eric
Author_Institution :
Univ. of California, Los Angeles, Los Angeles, CA, USA
Abstract :
We demonstrated direct nuclear delivery of macromolecules into live mammalian cells using the photothermal nanoblade. Pulsed laser triggered cavitation bubbles on a titanium thin film coated micropipette tip was utilized to puncture both the cell plasma and nuclear membranes followed by pressure controlled delivery of cargo into the nucleus. High plasmid expression (79-100%) in different cell types with maintained cell viability was achieved.
Keywords :
DNA; biomembranes; blades; bubbles; cavitation; cellular biophysics; drug delivery systems; drugs; gene therapy; laser applications in medicine; metallic thin films; molecular biophysics; nanomedicine; photothermal effects; titanium; DNA macromolecules; Ti; cell plasma; cell types; cell viability; direct nuclear delivery; high-plasmid expression; live mammalian cells; nuclear membranes; photothermal nanoblade; pressure controlled cargo delivery; pulsed laser triggered cavitation bubbles; titanium thin film coated micropipette tip; Biomembranes; DNA; Fluorescence; Lasers; Nanoscale devices; Plasmas; Titanium;
Conference_Titel :
Optical MEMS and Nanophotonics (OMN), 2013 International Conference on
Conference_Location :
Kanazawa
Print_ISBN :
978-1-4799-1512-5
DOI :
10.1109/OMN.2013.6659067
