Title :
Evaluation of transparent polyimide film as biological cell culture sheet with microstructures for biomedical engineering
Author :
Maenosono, Hirotaka ; Saito, Hiroshi ; Nishioka, Y.
Author_Institution :
Coll. of Sci. & Technol., Nihon Univ., Funabashi, Japan
Abstract :
Potentials of the transparent polyimide (PI) substrate were investigated for a possible biological cell culture sheet. Stromal marrow cells (OP9) were used to test the cell culture characteristics, and the OP9 cell culture was compared on three kinds of polymer films of transparent PI, parylene (PA), and polyetheretherketone (PEEK). Microtrenches with the width of 5 μm and the depth of 5 μm were fabricated using hot embossing technique on these films. The cultured cell of OP9 was observed on the transparent PI sheet and PA sheet, but not on PEEK sheet. The culture of OP9 cells were easily observed on the transparent PI sheet using an optical microscope.
Keywords :
biomedical engineering; biomedical materials; cellular biophysics; embossing; optical microscopy; polymer films; polymer structure; sheet materials; substrates; tissue engineering; transparency; PEEK sheet; biological cell culture sheet; biomedical engineering; cell culture characteristics; depth 5 mum; hot embossing technique; microstructures; microtrenches; optical microscopy; parylene; polyetheretherketone; size 5 mum; stromal marrow cells OP9; transparent polyimide film evaluation; transparent polyimide substrate; Cells (biology); Embossing; Microstructure; Polymers; Substrates;
Conference_Titel :
Optical MEMS and Nanophotonics (OMN), 2013 International Conference on
Conference_Location :
Kanazawa
Print_ISBN :
978-1-4799-1512-5
DOI :
10.1109/OMN.2013.6659088
