Atomic force microscope observation on CHO cell membrane after Electromagnetic pulses irradiation

Author

Zeng Li-Hua ; Li Yuan ; Miao Xia ; Wang Xiao-Wu ; Lang Hai-Yang ; Zhang Jie ; Li Yu-Rong ; Ren Dong-Qing ; Guo Guo-Zhen

Author_Institution

Dept. of Radiat. Med., Fourth Mil. Med. Univ., Xi´an

fYear

2008

fDate

2-5 Nov. 2008

Firstpage

1044

Lastpage

1047

Abstract

Atomic force microscope (AFM) has been used to visualize the morphological change on the surface of CHO cell membrane before and after Electromagnetic pulses (EMP)irradiation. The results showed that there were different sizes and shapes of membrane perforate (the width is 0.66-0.39 um, the depth is 392.95 nm) after 400 kV/m 200 pulses EMP irradiation. The numbers of cell membrane perforates were decreased with EMP field intensity and the pulses numbers decreasing. It is suggested that EMP irradiation can cause the membrane perforate of CHO cell directly. The numbers of cell membrane perforates were increased with EMP field intensity and the pulses numbers increasing. EMP induced membrane perforate may play a very important role in EMP bio-effects.

Keywords

atomic force microscopy; biological effects of fields; electromagnetic fields; CHO cell membrane; atomic force microscope observation; electromagnetic pulses irradiation bio-effects; hamster ovary; morphological change; size 0.66 mum to 0.39 mum; size 392.95 mm; Atomic force microscopy; Biomembranes; Cells (biology); EMP radiation effects; Electromagnetic forces; Pulse shaping methods; Shape; Space vector pulse width modulation; Surface morphology; Visualization; Atomic force microscope; CHO cell; cell membrane; electromagnetic pulse (EMP);

fLanguage

English

Publisher

ieee

Conference_Titel

Antennas, Propagation and EM Theory, 2008. ISAPE 2008. 8th International Symposium on

Conference_Location

Kunming

Print_ISBN

978-1-4244-2192-3

Electronic_ISBN

978-1-4244-2193-0

Type

conf

DOI

10.1109/ISAPE.2008.4735401

Filename

4735401