Title :
Quantitative analysis of living cells by digital holographic microscopy
Author :
Reese, Benjamin E. ; Barnett, Robert E. ; Alaoui-Ismaili, Mustapha ; Habibi, Emil ; Mann, Christopher J.
Author_Institution :
Dept. of Mech., Univ. of Tennessee, Knoxville, TN
Abstract :
Digital holography is applied to obtain noninvasive quantitative phase measurements from living cells. The complex wavefront can be recorded in just a single digital image allowing for critical real-time imaging. The vertical resolution of measurements is determined to be around 3 nanometers.
Keywords :
bio-optics; biological techniques; cellular biophysics; holographic interferometry; optical images; optical microscopy; biological microscopy; complex wavefront; digital holographic microscopy; digital image; holographic interference pattern; living cells; noninvasive quantitative phase measurement; quantitative analysis; real-time imaging; vertical resolution; Charge coupled devices; Charge-coupled image sensors; Focusing; Holography; Image reconstruction; Interference; Optical imaging; Optical microscopy; Phase measurement; Phase shifting interferometry;
Conference_Titel :
Biomedical Science & Engineering Conference, 2009. BSEC 2009. First Annual ORNL
Conference_Location :
Oak Ridge, TN
Print_ISBN :
978-1-4244-3837-2
DOI :
10.1109/BSEC.2009.5090485
