Title :
Evaluation of cell characteristics by opto-electrostatic micromanipulation
Author :
Nishioka, Masateru ; Katsura, Shinji ; Hirano, Ken ; Mizuno, Akira
Author_Institution :
Dept. of Ecological Eng., Toyohashi Univ. of Technol., Japan
Abstract :
Characteristics of a single cell of micrometer order can be easily studied using opto-electrostatic micromanipulation (OEMM) techniques. The authors present here the characteristics of Escherichia coli and Schizosaccharomyces pombe cells by trapping them with a YAG laser and rotating them under a high frequency alternating electric field. The intensity of the field was varied from 0.5×105 to 1.6×106 V/m (peak peak) and the frequency was changed from 5 kHz to 2 MHz. Characteristics of the cell rotation depends on frequency and intensity of the electric field. While the living cell has a peak critical rotation frequency (PCRF) at 1 MHz, that of a dead cell depends on the process of killing viz. thermal, chemical, ultraviolet or electric pulse. The measurement of the rotation rate will be utilized for investigating the dielectric properties of the individual cell
Keywords :
bioelectric phenomena; biological effects of fields; biological techniques; electric fields; electrostatics; laser beam applications; optical rotation; 1 MHz; 5 kHz to 2 MHz; Escherichia coli; HF AC electric field; Schizosaccharomyces pombe; YAG laser; cell rotation; dielectric properties; opto-electrostatic micromanipulation; peak critical rotation frequency; rotation rate measurement; Biomedical optical imaging; Chemical processes; Dielectric measurements; Dielectrophoresis; Electric variables measurement; Electrodes; Force measurement; Frequency; Optical feedback; Rotation measurement;
Conference_Titel :
Industry Applications Conference, 1995. Thirtieth IAS Annual Meeting, IAS '95., Conference Record of the 1995 IEEE
Conference_Location :
Orlando, FL
Print_ISBN :
0-7803-3008-0
DOI :
10.1109/IAS.1995.530459
