Title :
Optimized electrochemical detection of anti-cancer drug by carbon nanotubes or gold nanoparticles
Author :
Aliakbarinodehi, Nima ; De Micheli, Giovanni ; Carrara, Sandro
Author_Institution :
Integrated Systems Laboratory, École Polytechnique Fédérale de Lausanne (EPFL) Lausanne, Switzerland
fDate :
June 29 2015-July 2 2015
Abstract :
Nanostructured biosensors with the aim of electroactive cancer-drug detection were investigated. The aim of this work is improvement of the sensitivity and limit of detection of two differently nanostructured biosensors to find out the best choice for quantifying the concentration of etoposide, as a widely used electroactive cancer drug, in its therapeutic range. To this purpose etoposide concentrations, ranging from zero to 60 μM, were sensed at multi-walled carbon nanotube and gold nanoparticle functionalized bioelectrodes using cyclic voltammetry. The optimum scan rate for voltammetric experiments was found out equal to 70 mV s−1 and 130 mV s−1 for multi-walled carbon nanotube and gold nanoparticle based electrodes, respectively. For nanostructuring the electrodes, the optimum nanomaterial mass were experimentally obtained for multi-walled carbon nanotube and gold nanoparticle based electrodes equal to 20 μ g (4314 mm of additional electroactive surface area) and 104 g (6471 mm of additional electroactive surface area), respectively. Bioelectrodes produced based on this optimized configurations showed sensitivity of 0.98 ± 0.41 μA μM−1 cm−2 and 1.43 ± 0.26 μA μM−1 cm−2, and limit of detection of 1.52 ± 0.89 μM and 1.29 ± 0.48 μM for multi-walled carbon nanotube and gold nanoparticles based electrodes. Comparing the limit of detection achieved in this work with the therapeutic range of etoposide verifies the possibility of using both nanostructured bioelectrodes for etoposide detection. However, gold nanoparticle based electrodes exhibit better electrochemical improvements in terms of both sensitivity and limit of detection.
Keywords :
Biosensors; Carbon nanotubes; Drugs; Electrodes; Gold; Nanoparticles; Sensitivity; carbon nanotube; cyclic voltammetry; electroactive cancer-drug detection; electrochemical detection; gold nanoparticle; nanostructure properties;
Conference_Titel :
Ph.D. Research in Microelectronics and Electronics (PRIME), 2015 11th Conference on
Conference_Location :
Glasgow, United Kingdom
DOI :
10.1109/PRIME.2015.7251085