Variance in three-dimensional reconstructions from projections

Author

Penczek, Pawel A.

Author_Institution

Houston Med. Sch., Texas Univ., Houston, TX, USA

fYear

2002

fDate

2002

Firstpage

749

Lastpage

752

Abstract

Methods for estimation of variance for the three-dimensional (3D) reconstruction from projections are introduced. First, a Fourier space variance for a class of 3D reconstruction algorithms that involve interpolation is derived. It is used to construct a test for the validity of the single particle analysis process. Second, a real space variance/covariance for an arbitrary reconstruction algorithm is introduced. It is calculated using a bootstrap technique and used to investigate conformational variability of biological macromolecules.

Keywords

biological techniques; biology computing; covariance analysis; electron microscopy; image reconstruction; interpolation; low-temperature techniques; macromolecules; microorganisms; molecular biophysics; molecular configurations; proteins; 3D reconstruction algorithms; Fourier space variance; biological macromolecules; bootstrap technique; conformational variability; cryo-electron microscopy; interpolation; large macromolecular complexes; projections; protein-RNA complexes; proteins; real space variance/covariance; single particle analysis process; three-dimensional reconstructions; variance estimation; viruses; Biomedical imaging; Error correction; Image analysis; Image reconstruction; Image resolution; Noise level; Power capacitors; Proteins; Signal resolution; Signal to noise ratio;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging, 2002. Proceedings. 2002 IEEE International Symposium on

Print_ISBN

0-7803-7584-X

Type

conf

DOI

10.1109/ISBI.2002.1029366

Filename

1029366