Title :
Variance in three-dimensional reconstructions from projections
Author :
Penczek, Pawel A.
Author_Institution :
Houston Med. Sch., Texas Univ., Houston, TX, USA
Abstract :
Methods for estimation of variance for the three-dimensional (3D) reconstruction from projections are introduced. First, a Fourier space variance for a class of 3D reconstruction algorithms that involve interpolation is derived. It is used to construct a test for the validity of the single particle analysis process. Second, a real space variance/covariance for an arbitrary reconstruction algorithm is introduced. It is calculated using a bootstrap technique and used to investigate conformational variability of biological macromolecules.
Keywords :
biological techniques; biology computing; covariance analysis; electron microscopy; image reconstruction; interpolation; low-temperature techniques; macromolecules; microorganisms; molecular biophysics; molecular configurations; proteins; 3D reconstruction algorithms; Fourier space variance; biological macromolecules; bootstrap technique; conformational variability; cryo-electron microscopy; interpolation; large macromolecular complexes; projections; protein-RNA complexes; proteins; real space variance/covariance; single particle analysis process; three-dimensional reconstructions; variance estimation; viruses; Biomedical imaging; Error correction; Image analysis; Image reconstruction; Image resolution; Noise level; Power capacitors; Proteins; Signal resolution; Signal to noise ratio;
Conference_Titel :
Biomedical Imaging, 2002. Proceedings. 2002 IEEE International Symposium on
Print_ISBN :
0-7803-7584-X
DOI :
10.1109/ISBI.2002.1029366
