Effect of Oleic Acid on Triglycerides (TG) Accumulation and mRNA Level of PPARalpha and PPARgamma in Goose Primary Hepatocytes

In this study, the effect of oleic oil on cytoactive, triglycerides (TG) accumulation, lipids drop and mRNA expression of peroxisome proliferator-activated receptor alpha (PPARalpha) and peroxisome proliferator-activated receptor gamma ( PPARgamma ) was measured in primary goose hepatocytes. Results were as follows: 1.5 mmol/L oleic oil had an inhibiting effect on the cell viability; oleic oil could induce the goose hepatocytic steatosis and the increase of TG content and lipid droplet. Oleic oil all could increase the mRNA level of PPARalpha; 0.5 mmol/L oleic oil could increase the mRNA level of PPARgamma, but 1.0 mmol/L and 1.5 mmol/L oleic oil had no evident effect. Conclusion: in the model of oleic oil induced goose hepatocytic steatosis, the change of PPARalpha and PPARgamma expression level is favor of the balance of lipids metabolism in hepatocytes, and this may be related to the capability of keeping normal physiological function in the hepatic steatosis in waterfowl.