Microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery

Author

Du, Xiangving ; Yang, Yuesong ; Visage, Catherine Le ; Chen, Hunter H. ; DeJong, Roberi ; Qiu, Bensheng ; Wang, Danming ; Leong, Kam W. ; Hamper, UIrike M. ; Yang, Xiaoming

Author_Institution

Dept. of Radiol., Johns Hopkins Univ. Sch. of Medicine, Baltimore, MD, USA

fYear

2002

fDate

2002

Firstpage

989

Lastpage

992

Abstract

The purpose of this study was to test the feasibility of using a novel biodegradable microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery. We first encapsulated green fluorescent protein (GFP) gene into the microspheres and validated the echogenic property of GFP-plasmid/microspheres with intramuscular injection. Then, the GFP-plasmid/microspheres were transferred into the arteries of pigs via a catheter-based delivery procedure under ultrasound imaging. A strong echogenic signal reflected from the microspheres indicated the destination of genes delivered, whereas functional gene expression was confirmed by immunohistochemical examination. Microspheres can be feasible contrast agents/gene vectors in ultrasound imaging-based vascular gene delivery in vivo.

Keywords

biochemistry; biomedical ultrasonics; blood vessels; cardiovascular system; catheters; fluorescence; genetics; patient treatment; proteins; GFP-plasmid/microspheres; arteries; biodegradable microsphere; cardiovascular diseases; catheter-based delivery procedure; contrast agent; echogenic property; functional gene expression; gene destination; gene vector; green fluorescent protein gene; immunohistochemical examination; in vivo; intramuscular injection; pigs; strong echogenic signal; ultrasound imaging-based vascular gene delivery; vascular gene therapy; Arteries; Biodegradable materials; Catheters; Fluorescence; Gene expression; Gene therapy; Immune system; Monitoring; Polymers; Ultrasonic imaging;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging, 2002. Proceedings. 2002 IEEE International Symposium on

Print_ISBN

0-7803-7584-X

Type

conf

DOI

10.1109/ISBI.2002.1029429

Filename

1029429