Cell Biocompatibility of Porous Nano-Hydroxyapatite/Polyamide66 Composite Scaffolds for Bone Tissue Engineering

Author

Deng, Zhennan ; Mo, Anchun ; Ma, Jianfeng ; Xian, Suqin ; Zhang, Dafeng ; Liu, Jinsong

Author_Institution

Sch. & Hosp. of Stomatology, Wenzhou Med. Coll., Wenzhou, China

fYear

2009

fDate

17-19 Oct. 2009

Firstpage

1

Lastpage

5

Abstract

In this study, we prepared porous nano-hydroxyapatite/polyamide 66 (n-HA/PA66) composite scaffolds utilizing injection molding method. The morphology and macrostructure as well as mechanical strength of the scaffolds were characterized. Osteoblasts (OBs) were seeded on nHA/PA66 scaffolds as experiment group, and the same amount of OBs were seeded on n-HA/PA66 plate as control group. The OB/scaffold constructs were cultured for up to 18 days, then the adhesion, proliferation and osteogenic activity of OBs were observed by scanning electron microscope (SEM) and detected by alkaline phosphatase (ALP) activity, calcium content and osteocalcin (OC) concentration. The results show that the porous n-HA/PA66 composite scaffolds are biocompatible and have no negative effects on the OBs in vitro. The scaffolds fulfill the basic requirements of bone tissue engineering scaffold, and have the potential application in orthopedic, reconstructive and maxillofacial surgery.

Keywords

biomechanics; biomedical materials; bone; composite materials; orthopaedics; prosthetics; scanning electron microscopy; tissue engineering; adhesion; bone tissue engineering; cell biocompatibility; injection molding; macrostructure; maxillofacial surgery; mechanical strength; morphology; orthopedic surgery; osteoblast; porous nanohydroxyapatite/polyamide66 composite scaffolds; reconstructive surgery; scanning electron microscopy; Adhesives; Biological materials; Bone tissue; Calcium; Educational institutions; Mechanical factors; Orthopedic surgery; Polymers; Scanning electron microscopy; Tissue engineering;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics, 2009. BMEI '09. 2nd International Conference on

Conference_Location

Tianjin

Print_ISBN

978-1-4244-4132-7

Electronic_ISBN

978-1-4244-4134-1

Type

conf

DOI

10.1109/BMEI.2009.5305417

Filename

5305417