Exploration the load of molecular motors based on F0F1-ATPase

The potential of motor protein F₀F₁-ATPase is explored in the paper. A fluorescence probe F1300 labeled in inner chromatophores was used as a pH indicator for detecting proton flux driven by ATP synthesis in F₀F₁-ATPase. Furthermore, the F₁beta subunit of F₀F₁-ATPase was attached by a system of anti-beta antibody-biotin-avidin-biotin-second antibody (specificfor clenbuterol) as a load of molecular motors. The capturing mechanism was based on antibody-antigen reaction, while the detecting mechanism depended on fluorescence change responding to pH changes during rotary catalytic A TP synthesis process. The results shows that the activity of F₀F₁-ATPase-ATPase is affected with different loads.