Title :
Transfection of Human Embryonic Germ(EG) Cells with Human Cardiac alpha-Actin Promoter Driving pEGFP-N1
Author :
Hua, Jinlian ; Yang, Yuai ; Zhuang, Shuzhen ; Dou, Zhongying
Author_Institution :
Key Lab. of Mol. Biol. for Agric., Northwest A&F Univ., Yangling, China
Abstract :
In order to purify the cardiomyocytes derived stem cells, we constructed an expression vector, alpha -actin-pEGFP-N1 which consists of EGFP gene as reporter markers, and driven by human cardiac alpha-actin promoter, then transfected it into human embryonic germ cells(hEGCs) by Lipofectamine2000. Fluorescent observation and immunocytochemistry staining demonstrated that transfected hEGCs could differentiate into cardiomyocyte like cells and expressed GFP. The results suggest that this system may provide a model for the study of cardiogenesis and differentiation.
Keywords :
biochemistry; cardiology; cellular biophysics; fluorescence; Lipofectamine2000; alpha -actin-pEGFP-N1; cardiogenesis; cardiomyocytes; differentiation; expression vector; fluorescence; human cardiac alpha-actin promoter; human embryonic germ cells; immunocytochemistry staining; stem cells; transfaction; Cardiology; Cells (biology); Embryo; Heart; Humans; In vitro; Mice; Muscles; Pathogens; Stem cells;
Conference_Titel :
Biomedical Engineering and Informatics, 2009. BMEI '09. 2nd International Conference on
Conference_Location :
Tianjin
Print_ISBN :
978-1-4244-4132-7
Electronic_ISBN :
978-1-4244-4134-1
DOI :
10.1109/BMEI.2009.5305494
