Title :
Coupling Computers to Living Cells for Automated Knowledge Extraction
Author :
Callahan, Daniel E.
Author_Institution :
Live Cell Assays LLC, Martinez, CA, USA
Abstract :
A general method for coupling individual living cells to computers is presented. This method is called visual-servoing optical microscopy¿ (VSOM¿). Protocols, instrumentation, and software are described and experimental results are presented. During VSOM experiments, automated decisions are made as single cell responses to computer-applied stimuli are interpreted in real-time. Well-annotated live cell responses are stored in distributed VSOM databases in real-time. This closed-loop method enables automated extraction of knowledge from individual living cells.
Keywords :
biology computing; cellular biophysics; distributed databases; knowledge acquisition; living systems; optical microscopy; visual servoing; VSOM; automated knowledge extraction; computer-applied stimuli; coupling computers; distributed VSOM databases; living cells; visual-servoing optical microscopy; Computer industry; Digital images; Fluorescence; Instruments; Operating systems; Optical microscopy; Protocols; Software prototyping; System software; Visual databases; biologic assays; biomedical informatics; cells; computer vision systems; computer-assisted image interpretation; fluorescence microscopy; microscopy; visual servoing;
Conference_Titel :
eScience, 2008. eScience '08. IEEE Fourth International Conference on
Conference_Location :
Indianapolis, IN
Print_ISBN :
978-1-4244-3380-3
Electronic_ISBN :
978-0-7695-3535-7
DOI :
10.1109/eScience.2008.152
