Title :
Attachment of heavy metal to DNA molecules for rapid analysis
Author :
Masudur, Rahman Md ; Komatsu, Jun ; Katsura, Shinji ; Mizuno, Akira
Author_Institution :
Dept. of Ecological Eng., Toyohashi Univ. of Technol., Japan
Abstract :
Labeling of DNA has been studied using base-specific Rhodium (II) acetate [Rh2(O2CCH3)4] (Rh1) compound. First, DNA and Rh1 were mixed and the binding reaction was checked by electrophoresis of the complex. The result showed that some of DNA molecules were labeled by the Rh1. AFM observation also showed some overlapped DNA molecules. It suggests that DNA molecules could be cross-linked by Rh1 on the overlapped points.
Keywords :
DNA; atomic force microscopy; bioelectric phenomena; electrophoresis; molecular biophysics; AFM; DNA labeling; DNA molecules; DNA-Rh1 binding; base-specific Rhodium acetate; electrophoresis; heavy metal attachment; Atomic force microscopy; Biochemistry; DNA; Drugs; Electrokinetics; Ethanol; Inorganic materials; Nanobioscience; Sequences; Transmission electron microscopy;
Conference_Titel :
Industry Applications Conference, 2005. Fourtieth IAS Annual Meeting. Conference Record of the 2005
Print_ISBN :
0-7803-9208-6
DOI :
10.1109/IAS.2005.1518418
