Protein Adsorption and Biocompatibility of Porous Silk Fiboin Films

Author

Sun Zi Ling ; Bai Lun ; Guan Guo Ping ; Zhan Kui Hua ; Dai Hong Qin

Author_Institution

Coll. of Textile & Clothing Eng., Soochow Univ., Suzhou, China

fYear

2009

fDate

17-19 Oct. 2009

Firstpage

1

Lastpage

4

Abstract

Silk fibroins are widely used in skin wound healing and tissue engineering scaffolds. This study sought to observe proteins adsorption and biocompatibility. Bradford protein assay revealed that the amount of adsorbed proteins was significantly less than that on PVA. The Sodium Dodecyl Sulfate Polyacrylamidege Electrophoresis (SDS-PAGE) indicated that more proteins adsorbed onto PVA than that PSFFs. Identification of adsorbed proteins by Western blotting revealed that there was lesser amount of fibrinogen on PSFFs than on PVA. Histologic observation after 10 days PSFFs implantation in shin and muscle of rat showed that some capillaries and microvessels grew in PSFFs, PSFFs and peritissues were mixed with each other, many fibroblasts, myocytes and lipocytes could be observed in PSFFs due to good biocompatibility.

Keywords

adsorption; biomedical materials; cellular biophysics; porous materials; proteins; tissue engineering; Bradford protein assay; SDS-PAGE; Sodium Dodecyl Sulfate Polyacrylamidege Electrophoresis; Western blotting; biocompatibility; capillaries; fibroblasts; histologic observation; lipocytes; microvessels; myocytes; peritissues; porous silk fiboin films; protein adsorption; rat muscle; rat shin; skin wound healing; tissue engineering scaffolds; Adhesives; Biological materials; Biomedical materials; Biomembranes; Educational institutions; Electrokinetics; Plasma chemistry; Plasma temperature; Protein engineering; Sun;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics, 2009. BMEI '09. 2nd International Conference on

Conference_Location

Tianjin

Print_ISBN

978-1-4244-4132-7

Electronic_ISBN

978-1-4244-4134-1

Type

conf

DOI

10.1109/BMEI.2009.5305691

Filename

5305691