Title :
Separation imaging of DNA fragments in poly (dimethylsiloxane) microchip
Author :
Liu, Changchum ; Cui, Dafu
Author_Institution :
State Key Lab. of Transducer Technol., Chinese Acad. of Sci., Beijing, China
Abstract :
Poly (dimethylsiloxane) (PDMS) had been extensively used as excellent microchip material due to its particular advantages. In this paper, Laser-induced fluorescence detection system for visualizing microchip electrophoresis had been constructed and a series of electrophoresis operation of DNA fragments labeled by SYBR Green I were imaged by charge coupled device (CCD) camera. We investigated the influence of the sieving matrix concentration and the electric field strength on the formation of sample injection plug. PCR markers were well separated in PDMS microchip.
Keywords :
CCD image sensors; DNA; electrophoresis; fluorescence; microchip lasers; microfluidics; DNA fragment; Laser-induced fluorescence detection system; PDMS microchip; charge coupled device camera; microchip electrophoresis; polydimethylsiloxane microchip; Charge coupled devices; Charge-coupled image sensors; Costs; DNA; Electrokinetics; Filters; Fluorescence; Laser excitation; Microchip lasers; Microscopy;
Conference_Titel :
Information Acquisition, 2004. Proceedings. International Conference on
Print_ISBN :
0-7803-8629-9
DOI :
10.1109/ICIA.2004.1373352
