DocumentCode
2205699
Title
Separation imaging of DNA fragments in poly (dimethylsiloxane) microchip
Author
Liu, Changchum ; Cui, Dafu
Author_Institution
State Key Lab. of Transducer Technol., Chinese Acad. of Sci., Beijing, China
fYear
2004
fDate
21-25 June 2004
Firstpage
205
Lastpage
207
Abstract
Poly (dimethylsiloxane) (PDMS) had been extensively used as excellent microchip material due to its particular advantages. In this paper, Laser-induced fluorescence detection system for visualizing microchip electrophoresis had been constructed and a series of electrophoresis operation of DNA fragments labeled by SYBR Green I were imaged by charge coupled device (CCD) camera. We investigated the influence of the sieving matrix concentration and the electric field strength on the formation of sample injection plug. PCR markers were well separated in PDMS microchip.
Keywords
CCD image sensors; DNA; electrophoresis; fluorescence; microchip lasers; microfluidics; DNA fragment; Laser-induced fluorescence detection system; PDMS microchip; charge coupled device camera; microchip electrophoresis; polydimethylsiloxane microchip; Charge coupled devices; Charge-coupled image sensors; Costs; DNA; Electrokinetics; Filters; Fluorescence; Laser excitation; Microchip lasers; Microscopy;
fLanguage
English
Publisher
ieee
Conference_Titel
Information Acquisition, 2004. Proceedings. International Conference on
Print_ISBN
0-7803-8629-9
Type
conf
DOI
10.1109/ICIA.2004.1373352
Filename
1373352
Link To Document