Title :
Monitoring Cerebral Hemodynamics Following Optogenetic Stimulation via Optical Coherence Tomography
Author :
Atry, Farid ; Frye, Seth ; Richner, Thomas J. ; Brodnick, Sarah K. ; Soehartono, Alana ; Williams, Julia ; Pashaie, Ramin
Author_Institution :
Electr. Eng. & Comput. Sci. Dept., Univ. of Wisconsin-Milwaukee, Milwaukee, WI, USA
Abstract :
In this article, spectral domain optical coherence tomography is used to measure the hemodynamic response induced by optogenetic stimulation in the somatosensory cortex of transgenic mice. By analyzing the 3-D angiograms and Doppler measurements produced by coherence tomography, we observed significant increase in blood flow as a result of increased vessel diameter and blood velocity following optical stimulation of cortical neurons. Such distinct responses were not observed in control experiments where the brain of wild-type mice were exposed to the same light pulses.
Keywords :
Doppler measurement; biomedical optical imaging; blood vessels; brain; cellular biophysics; genetics; haemodynamics; optical tomography; patient monitoring; radiation therapy; somatosensory phenomena; 3D angiogram analysis; Doppler measurements; brain; cerebral hemodynamic monitoring; cortical neurons; optogenetic stimulation; somatosensory cortex; spectral domain optical coherence tomography; transgenic mice; vessel diameter; Biomedical optical imaging; Blood; Laser beams; Mice; Optical imaging; Stimulated emission; Functional monitoring and imaging; Laser Doppler velocimetry.; Medical and biological imaging; Optical coherence tomography; Optogenetics; laser Doppler velocimetry; medical and biological imaging; optical coherence tomography; optogenetics;
Journal_Title :
Biomedical Engineering, IEEE Transactions on
DOI :
10.1109/TBME.2014.2364816
