Electrochemical biosensor array for liver diagnosis using a silanization technique on nano-porous silicon electrode

Cholesterol, bilirubin and ALT/AST present in the serum are often used as biomarkers for liver diseases. For this study, we describe our biosensor array system consisting of cholesterol, bilirubin and glutamate sensors. We determined that porous silicon (PS) layers formed on each working electrode greatly increased the effective surface area. The electrodes in the sampling wells minimized a cross-interference effect to permit multiple sampling by immobilization of the enzymes using a silanization technique. The device sensitivities observed were 0.2656 muA/mM for cholesterol, 0.15354 mA/mM for bilirubin, 0.13698 muA/(U/l) for ALT and 0.45439 muA/(U/l) for AST.