Title :
Optimization of laser-induced fluorescence detection system for microfluidic chip
Author :
Shi, Pan ; Hu, Xiaoming ; Li, Qin ; Deng, Yulin
Author_Institution :
Dept. of Biomed. Eng., Beijing Inst. of Technol., Beijing
Abstract :
Laser-induced fluorescence (LIF) detection is widely utilized in microfluidic chip due to its high sensitivity and selectivity. In this paper, a LIF detection system for the microscope chip based on a 473 nm laser diode is described. To improve the performance of the detection system, the confocal configuration is adopted in our experiment. Some factors that are important to the signal-to-noise ratio (SNR) of the confocal system, such as the choice of microscope objective, effect of excitation source, the size of pinhole and the performance of the PMT detection units are presented and explained in this paper.
Keywords :
bioMEMS; biomedical measurement; biomedical optical imaging; fluorescence; lab-on-a-chip; laser applications in medicine; measurement by laser beam; microfluidics; optical microscopy; photomultipliers; semiconductor lasers; LIF detection; PMT detection units; SNR; confocal configuration; excitation source; laser diode; laser-induced fluorescence detection system; microfluidic chip; microscope chip; pinhole size; signal-to-noise ratio; wavelength 473 nm; Biomedical optical imaging; Diode lasers; Fluorescence; Laser beams; Laser excitation; Microfluidics; Microscopy; Optical filters; Optical sensors; Voltage;
Conference_Titel :
Information Technology and Applications in Biomedicine, 2008. ITAB 2008. International Conference on
Conference_Location :
Shenzhen
Print_ISBN :
978-1-4244-2254-8
Electronic_ISBN :
978-1-4244-2255-5
DOI :
10.1109/ITAB.2008.4570634
